issue date：2016-3-31 15:35:44 visit frequency：3215
Dual wavelength is used in many microplate-based applications to reduce optical interference caused by scratches, fingerprints or other matter that absorb light equally at both wavelengths. For example, many investigators prefer to read microplate-based assays with lids or membrane seals in place to reduce biohazards, as well as evaporation. As a result of using lids, condensation may collect on the lid during the assay process. To illustrate the benefits of dual wavelength in these types of applications, XF performed experiments showing what effect on results a plate lid, with or without condensation, can have when readings are taken at single vs. dual wavelength. The experiments demonstrated that when the plate is read at two wavelengths and the difference in optical densities is computed, this technique adequately compensated for these effects.
|
Material |
Crystal Quartz |
|
Parallelism |
<1 arc second |
|
Diameter Tolerance |
+0.0/-0.1 |
|
Surface Quality |
20/10 (Scratch/Dig) |
|
Retardation Tolerance |
λ/300 |
|
Wavefront Distortion |
λ/8@632.8nm |
|
Clear Aperture |
＞90% |
|
AR Coating |
<0.2% Wavelength |
|
Damage Threshold |
500MW/cm2 in 5 ns pulse |
|
Waveength Range |
240-2100nm |
|
Diameter(mm) |
Phase Retardation |
Part Number |
|
|
10.0 |
λ@1064nm +λ/2@532nm |
WPDW101 |
|
|
12.7 |
λ@1064nm +λ/2@532nm |
WPDW 111 |
|
|
15.0 |
λ@1064nm +λ/2@532nm |
WPDW 121 |
|
|
20.0 |
λ@1064nm +λ/2@532nm |
WPDW 131 |
|
|
25.4 |
λ@1064nm +λ/2@532nm |
WPDW 141 |
|
|
30.0 |
λ@1064nm +λ/2@532nm |
WPDW 151 |
